Figure 2
Figure 2. Vegfc deletion at E7.5 leads to anemia; FL sinusoidal vasculature or hepatocytes are not affected. (A) Representative microscopic images of Wright-Giemsa–stained PB smears from E15.5 embryos and quantification of the ratio of nucleated red blood cells (RBCs)/enucleated RBCs (n = 4-8 per group; mean ± SEM; *P < .05). Bar represents 10 μm. (B) PB erythrocyte and hemoglobin analysis of E15.5 VciΔR26 embryos (n = 3-4 per group, mean ± SEM; *P < .05; ***P < .001). (C) Flow cytometric analysis of FLs. Note a decrease of erythroid cells (Ter-119+CD45−) in VciΔR26 and WT FLs (n = 4-5 per group; mean ± SEM; *P < .05). (D-E) Staining and quantification of LYVE1+ sinusoidal ECs, E-cadherin+ hepatocytes, and von Willebrand factor (vWF)+ megakaryocytes (n = 3 per group; mean ± SEM; P > .05). Bar represents 200 μm.

Vegfc deletion at E7.5 leads to anemia; FL sinusoidal vasculature or hepatocytes are not affected. (A) Representative microscopic images of Wright-Giemsa–stained PB smears from E15.5 embryos and quantification of the ratio of nucleated red blood cells (RBCs)/enucleated RBCs (n = 4-8 per group; mean ± SEM; *P < .05). Bar represents 10 μm. (B) PB erythrocyte and hemoglobin analysis of E15.5 VciΔR26 embryos (n = 3-4 per group, mean ± SEM; *P < .05; ***P < .001). (C) Flow cytometric analysis of FLs. Note a decrease of erythroid cells (Ter-119+CD45−) in VciΔR26 and WT FLs (n = 4-5 per group; mean ± SEM; *P < .05). (D-E) Staining and quantification of LYVE1+ sinusoidal ECs, E-cadherin+ hepatocytes, and von Willebrand factor (vWF)+ megakaryocytes (n = 3 per group; mean ± SEM; P > .05). Bar represents 200 μm.

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