CD19-CAR–modified T_SCM have a transcriptome profile similar to their naturally occurring counterpart. (A) Hierarchical clustering of CD19-CAR–modified standard and T_SCM-enriched products and naturally occurring CD8⁺ T-cell subsets from GSE23321 performed using a 900 gene list from Gattinoni et al.⁹  Red and blue colors indicate increased and decreased expression of 900 differentially regulated genes described by Gattinoni et al.⁹  Each column represents a sample and each row, a gene. (B) Principal component analysis (PCA) of the 900 differentially expressed genes described by Gattinoni et al⁹  in CD19-CAR–modified standard and T_SCM-enriched products and naturally occurring CD8⁺ T-cell subsets from Gattinoni et al.⁹  (C). Gene set enrichment analysis (GSEA) on transcriptomes of CD19-CAR–modified standard and T_SCM-enriched products using genes upregulated or downregulated in T_SCM relative to T_EM retrieved from Gattinoni et al⁹  as gene sets. (D) Schematic representation of the glycolytic pathway. In blue font, glycolytic enzymes and molecules whose genes were downregulated in CD19-CAR–modified T_SCM-enriched cells compared with standard products (P < .05). In gray font, glycolytic enzymes not differentially expressed. DHAP, dihydroxyacetone phosphate; S/N, signal/noise.