Antigen-specific immune responses in patients with AML exhibiting ecto-CRT⁺vs ecto-CRT⁻blasts. (A-B) Representative dot plots (A) and quantitative data (B) of IFN-γ–secreting CD45⁺CD3⁺CD4⁺ T cells and CD45⁺CD3⁺CD8⁺ T cells from 10 HD or 15 CRT^Hi vs 15 CRT^Lo patients with AML before induction chemotherapy (Prior, B) and at reestablishment of normal hematopoiesis (Recovery, A-B), upon exposure of the corresponding PBMCs to peptide mixture spanning BIRC5, CCNB1, PRAME, or WT1. Box plots: lower quartile, median, upper quartile; whiskers, minimum value, maximum value.