Clonal biases and variance of biases. (A) The ratio of the fractional contributions of each clone to monocytes (Mono) versus granulocytes (Gr) at each time point is mapped over time for ZH33 (top). All clones are shown; however, larger clones have darker lines and are plotted in the foreground. Smaller clones are shown with lighter lines in the background. Small (light) clones are more likely to appear highly biased because of sampling constraints. Note that clones are overall unbiased in monocyte versus granulocyte production, and individual clone bias is generally stable over time. The variance of these individual clonal biases up to the specified time point for Mono/Gr are shown at the bottom, demonstrating a marked stabilization of bias over time. The precise definition of bias is given in the supplemental data. (B) The same analyses as in (A) are shown for B cells versus granulocytes (B/Gr). Clones may be biased in production of B cells versus granulocytes, but individual clone bias is generally stable over time. Larger clones generally have smaller variance. (C) The same analyses as in (A) are shown for T cells versus B cells. Clones may be highly and stably biased toward T-cell (and away from B-cell) production and are less stable over time (vertical movement in bottom graph). Larger clones do not necessarily have smaller variance in this bias comparison, in contrast to the comparisons shown in (A) and (B).