sIgM levels or signaling capacity and DNA methylation maturation status in CLL. DNA methylation profiling of 18 selected regions was determined by MassARRAY in 57 CLL samples and a MMS was calculated. (A) The associations between MMS and IGHV status (U-CLL vs M-CLL), sIgM level status (sIgM MFI high vs sIgM MFI low), and signaling capacity (sIgM high-signaler vs low-signaler) are represented. Horizontal bars indicate mean values. Statistical analysis was performed using the Mann-Whitney test (2 tailed, 95% CI). (B) Correlation analysis between MMS and sIgM levels and signaling capacity in U-CLL and in M-CLL. Statistical analyses were performed using the Spearman rank correlation test. In all panels, empty symbols represent CLL with isolated del13q or negative FISH; black filled symbols represent CLL with either +12 or del(11q) or del(17p) or NOTCH1ΔCT or SF3B1 mutation. Square symbols represent IGHV3-21+ CLL. NS, not significant.
