Analysis of hematopoietic stem and progenitor subsets in JAK2-Ex12 transgenic mice. (A) Bar graphs shows the average frequency of LT-HSC (Lin⁻Kit⁺Sca-1⁺CD150⁺CD48⁻), ST-HSC (Lin⁻Kit⁺Sca-1⁺CD150⁺CD48⁺), MPP (Lin⁻Kit⁺Sca-1⁺CD150^lo CD48⁺), and LSK in total BM (left) and spleen (right) of indicated mice (n = 5-6 mice per each genotype). (B) Average frequency of myeloid precursors LK, CMP (Lin⁻Kit⁺Sca-1⁺ CD34⁺ FcγRII/III^lo), GMP (Lin⁻Kit⁺Sca-1⁺ CD34⁺ FcγRII/III⁺), and MEP (Lin⁻Kit⁺Sca-1⁺ CD34⁻ FcγRII/III^lo) in total BM (left) and spleen (right) of indicated mice (n = 5-6 mice per each genotype). (C) Average frequency of MK and erythroid committed subsets, Pre-GM (Lin⁻Kit⁺Sca-1⁻CD41^−/+CD150⁻CD105⁻), Pre-MegE (Lin⁻Kit⁺Sca-1⁻CD41^−/+ CD150⁺CD105⁻), MkP (Lin⁻Kit⁺Sca-1⁻CD41⁺CD150⁺), Pre–erythroid CFU (CFU-E) (Lin⁻Kit⁺Sca-1⁻CD41⁻CD150⁺CD105⁺), and CFU-E (Lin⁻Kit⁺Sca-1⁻CD41⁻CD150^loCD105⁺) in total BM (left) and spleen (right) of indicated mice (n = 5-6 mice per each genotype). Mice were analyzed 16 weeks after pIpC injection. (D) Analysis of colonies grown in semisolid media. Numbers on Y-axis indicate the colony counts. (E) Serum Epo levels. (F) Plasma Tpo levels (n = 6 mice per genotype). All data are presented as mean ± SEM. One-way or two-way ANOVA with subsequent Bonferroni post-test was used. *P < .05; **P < .01; ***P < .001. CMP, common myeloid progenitors; GMP, granulocyte-monocyte progenitors; HSC, hematopoietic stem cell; LK, Lin⁻cKit⁺; LSK, Lin⁻Sca-1⁺cKit⁺; LT-HSC, long-term HSC; MEP, MK-erythrocyte progenitors; MkP, MK progenitors; MPP, multipotent progenitors; Pre-GM, pre–granulocyte-monocyte progenitors; Pre-MegE, Pre–megakaryocyte-erythroid progenitors; ST-HSC, short-term HSC.