Drastically reduced numbers of splenic DCs and BM progenitor cells in Gata2 knockout mice. (A) Gata2 deletion was confirmed via PCR analysis of genomic DNA isolated from total BM cells of mice injected with tamoxifen or corn oil. (B) Relative levels of Gata2 mRNA in BM cells of mice injected with tamoxifen or corn oil. Data are expressed as means ± SDs (n = 4). *P < .01. (C) Percentages of splenic DC populations (pDCs and cDCs) among splenocytes from Gata2^f/f/ER-Cre mice injected with tamoxifen or corn oil were determined using flow cytometry. Data are expressed as means ± SDs (n = 4). *P < .01. (D) Immunohistochemical analysis of spleens from Gata2^f/f/ER-Cre mice treated with tamoxifen or corn oil. Sections were stained with an antibody against CD11c (CD11c⁺ cells are brown). Scale bar, 50 μm. (E) Percentages of BM progenitor cells from Gata2^f/f/ER-Cre mice injected with tamoxifen or corn oil were determined using flow cytometry. Data are expressed as means ± SDs (n = 4). All differences are significant (P < .005).