GATA2 negatively regulates the transcriptional activity of Gata3. (A) A quantitative ChIP analysis of GATA2 chromatin occupancy at Gata3 locus in CMPs (left) and EML cells (right) (means ± SDs, n = 3). The necdin promoter and Gata2 +9.5 kb were used as negative and positive controls, respectively.²⁷  (B) Transcriptional activity through the Gata3 +190-kb site assessed via luciferase assay. The Gata3 promoter (Gata3 prom), Gata3 +190-kb region + Gata3 promoter (Gata3 +190 kb + prom), or GATA sequence-deleted Gata3 +190-kb region + Gata3 promoter (Gata3 +190 kb deletion + prom) was fused to a luciferase reporter gene and transiently transfected into EML cells (means ± SDs; n = 5). *P < .05.