Activating, blocking, and neutral antibodies toward LFA-1 affect binding of Jβ2/LFA-1 cells or T blasts to ICAM-1. (A,B) Static adhesion test to ICAM-1. Jβ2.7/LFA-1 cells (A) or T blasts (B) were activated with the chemokine SDF-1α or activating antibodies or with SDF1-α alone or together with blocking or neutral antibodies. Cells were allowed to bind to an ICAM-1-coated surface, unbound cells washed off, and bound cells counted from 6 screens in triplicate. Amounts of bound cells and standard deviations are shown. **P < .01.