Figure 2
Treatment of Jβ2/LFA-1 cells or T blasts with activating or blocking antibodies leads to transdominant inhibition of α4β1. Jβ2.7 or Jβ2.7/LFA-1 cells (A) or naive T cells or T blasts (D) were treated with SDF1-α, indicated LFA-1 antibodies, or left untreated. (B) Jβ2.7/LFA-1 cells were treated with different concentrations of the LFA-1 blocking antibody 7E4 or the neutral antibodies CBR LFA-1/7 or TS2/4. (C) Jβ2.7/LFA-1 cells were treated with the 7E4 antibody or an equal molar amount of the Fab fragment of the antibody. Adhesion to VCAM-1 under flow was quantified from 6 screens in triplicate. Amounts of bound cells and standard deviations shown. *P < .05; **P < .01, compared with untreated control of the same cell type.
