Treatment of Jβ2.7/LFA-1 cells or T blasts with activating or blocking antibodies results in phosphorylation of β₂, PLCβ₃, and PLCγ₁. Jβ2.7/LFA-1 cells (A) or T blasts (B) were treated with SDF1-α, antibodies or left untreated. Cells were lysed and analyzed by western blotting, with the specific antibodies indicated to the right. (C) T blasts were allowed to settle on poly-l-lysine, were treated with SDF-1α, the indicated antibodies, or left untreated. Cells were fixed and stained with phalloidin or the β₂/pThr-758 antiserum. Scale bar represents 10 μM.