Representative IHC staining and protein level associations based on capture sequencing rearrangement status for CD274 (PDL1) and PDCD1LG2 (PDL2). (A) In case CS29, only macrophages stained for CD274, whereas the majority of tumor cells express PDCD1LG2 due to a focal amplification of the PDCD1LG2 gene locus. CS54 harbored an inversion displacing the 3′ end of CD274 and disrupting the coding sequence of PDCD1LG2. Concordantly, the tumor cells display strong positivity for CD274 and remain negative for PDCD1LG2. CS49 harbors a CBR2 translocation and shows a similar staining pattern as described for CS29. Scale bar, 25 μm. (B) Cases were considered rearranged if they had a CBR translocation or a focal amplification of the gene region. The bold line represents the median, and the box denotes the interquartile range. Individual cases are represented by points. P value was calculated using an exact Wilcoxon-Mann-Whitney test.