Engineered DOHH-2 cell lines and Jurkat CD69 expression following coculturing. (A) PDCD1LG2 surface expression on DOHH-2 cell lines stably transduced with wild-type PDCD1LG2 or PDCD1LG2-IGHV7-81 is provided compared to DOHH-2 cells transduced with empty vector. (B) CD69 expression in Jurkat cells following stimulation and coculturing for 28 hours with transduced DOHH-2 cell lines. Coculturing was performed in triplicate, using a ratio of 1:5 T:B cells. Significance was assessed using a 2-tailed, paired Student t test. **P < .01. NS, not significant. (C) Representative distribution of CD69 among Jurkat cells cocultured with empty vector, PDCD1LG2 wild-type expressing, or PDCD1LG2-IGHV7-81 expressing DOHH-2.