CX3CR1 reduces Ly6C^highmonocyte release toward the peritoneal cavity during LPS-mediated peritonitis. Kinetics of Ly6C^high and Ly6C^low monocyte mobilization in the spleen (A), the lungs (B), and the peritoneal cavity (C) after intraperitoneal injection of 100 ng/kg LPS in MacBlue×Cx3cr1^gfp/+ (WT) and MacBlue×Cx3cr1^gfp/gfp mice (Cx3cr1^−/−). Representative dot plots show the gating strategy to define Ly6C^high (red gate) and Ly6C^low monocytes (blue gate); percent ± SD are depicted. Graphs represent mean ± SEM of the absolute number per milligram of spleen or lungs and in total after peritoneal cavity lavage (n = 8-13 mice per group and per time point from at least 3 independent experiments. Kruskal-Wallis with Dunn’s multicomparisons test is performed; the significance for each time point compared with untreated mice [t0] are indicated in colored *. Significance between mouse strains are indicated by black * for each time point, and Mann-Whitney test is performed). *P < .05; **P < .01; ***P < .001. AM, alveolar macrophages; NK, natural killer cells.