A pharmacological library screen leading to the identification of 3 compounds that stimulate CD19 gene transcription in cHL cells. (A) Firefly luciferase–based CD19 reporter readout validation of 55 preselected compounds in 3 cHL reporter cell lines. Cells were incubated with the respective chemicals at 10 μM for 48 hours or left untreated (UT). Fold-induction values >2 are marked in red (see color coding). Numeric codes of the compounds are listed at the bottom, highlighted in yellow or blank groups indicating similar structure. Blue boxes mark 6 groups of chemicals, comprising 13 compounds (see supplemental Figure 3D), as effective in all 3 cHL cell lines. (B) Stimulation of CD19 transcription in cHL cell lines after a 48-hour exposure to 10 μM of compound 27, 40, and 49 (as marked in panel A), detected by RQ-PCR (presented as mean ± SD; *P < .05). (C) Structural formulas of screen-identified chemical compounds 27, 40, and 49.