Imaging intravascular coagulation in sepsis. Representative SD-IVM images of the liver microcirculation in untreated (A-C), endotoxemic (D-F), E coli–infected (G), and S aureus–infected (H) mice. Thrombin probe fluorescence is shown in green (note the level of background autofluorescence of the liver parenchyma shown in panel A), and platelets are shown in blue (AF647 anti-CD49b). Bar represents 50 μm. (I) Quantitative analysis of thrombin probe fluorescence within the liver microcirculation of untreated control mice, LPS-treated mice, or LPS-treated mice that received a direct thrombin inhibitor (argatroban). Data are shown as mean ± SEM. **P < .01; N = 3-6 mice per group.