Reducing NET production in PAD4-deficient mice reduces intravascular coagulation. Representative SD-IVM images of the liver microcirculation of wild-type (A, PAD4^+/+) and PAD4^−/− (B) mice 4 hours after LPS administration. Thrombin probe fluorescence is in green, NETs are shown in red (AF555 anti-H2Ax), and neutrophils are shown in blue (AF647 anti-Gr1). Bars represent 50 μm. Quantitative analysis of NETs (C-E) and thrombin probe fluorescence (F-H) within the liver microvasculature of wild-type (PAD4^+/+) and PAD4^−/− mice after administration of LPS, E coli infection, or S aureus infection. Data are represented as mean ± SEM. **P < .01; N = 3-6 mice per group.