Blocking NETs-induced intravascular coagulation restores microvascular perfusion. Representative SD-IVM images of the liver microcirculation of endotoxemic wild-type (PAD4^+/+) (A-B) and PAD4^−/− (C-D) mice. Thrombin probe activity is shown in green. Mice were then injected with AF647-albumin (blue) as a contrast material to identify perfused versus occluded vessels. Bars represent 50 μm. The proportion of occluded vessels was quantified per field of view in (E) endotoxemic wild-type (PAD4^+/+) versus PAD4^−/− mice and (F) endotoxemic mice treated with IV DNase versus vehicle control. (G) Serum lactate levels were quantified in blood samples from septic mice (24 hours after i.p. infection with E coli) treated with IV DNase versus vehicle control. Data are represented as mean ± SEM. **P < .01, *P < .05; N = 3-6 mice per group.