Mouse carotid artery thrombosis model. FXII-deficient C57Bl/6 mice were infused with 100 μL of PBS (C) or 100 μl of PBS containing 25 μg of FXII-WT, FXII-R353A, FXII-T, or FXII-S544A. Thrombus formation was induced by application of 2 pads saturated with 5% FeCl₃ to opposite sides of the carotid artery for 3 minutes. Flow through the artery was recorded for 30 minutes. The percentages of animals with occluded arteries 30 minutes after FeCl₃ application are shown in the bar graph (n = 9 for each bar). Representative plasma samples from test mice were analyzed by western blot to make certain that FXII was still in the circulation at the end of the study. Each number indicates a separate animal. C, FXII control; DP, FXII-deficient mouse plasma; NP, normal mouse plasma.