Mof-deficient embryos start manifesting hematopoietic defects at E17.5. (A) Representative photographs of Vav1-cre;Mof^f/f, Vav1-cre;Mof^f/+, and Mof^f/f embryos at E17.5. (B) PCR analysis illustrating Mof excision in fresh CD45.2⁺ FL cells. A representative image is shown. (C) Western blot showing global H4K16ac, H4, and actin in fresh CD45.2⁺ FL cells. (D) Total live FL cell count. (E) The absolute number of cells at various stages of differentiation within the erythroid lineage in E17.5 FLs as measured by FACS. (F) Total number of live CD45.2⁺ FL cells. (G) The absolute number of mature B cells (B220⁺), myeloid cells (GR1⁺), and T cells (CD3⁺) in E17.5 FL cells as measured by FACS. (H) The absolute number of MPs, LSKs, and HSCs (CD150⁺/CD48^–) as measured by FACS. (I) Day 10 of CFU assay of fresh FL cells derived from E17.5 Vav1-cre;Mof^f/f, Vav1-cre;Mof^f/+, and Mof^f/f embryos. Bar graph indicates mean number of colonies per dish after 10 days. In all, 20 000 cells were plated per dish. Each groups contains 3 biological replicates. (J) Shown is the mean percentage of the various colony types relative to all colonies counted per dish per genotype. Error bars represent SD of mean. Significance is shown for comparing Vav1-cre;Mof^f/f and Vav1-cre;Mof^f/+ to Mof^f/f. *P < .05; **P < .01.