PBMC staining and clustering. PBMCs from AA and HDs were clustered using 34 surface and intracellular markers as our panel 1 (supplemental Table 1). Intact cells were gated based on Ir-191 and event length, followed by Ir-191 and Ir-193 gating. Viable cells were selected based on CD45 expression and negativity for Rh. All FCS files were first normalized using control beads and analyzed using Cytobank web-based software (see “Methods”). CD4⁺ and CD8⁺ T and B cells clustered together in both AA and HDs. Figures are representative of 16 AA and 5 HD samples.