BCAP differentially regulates myeloid and lymphoid cell development and/or homeostasis. (A) Representative flow cytometry plots identifying CD45.1⁺ and CD45.2⁺ inflammatory (Infl.) monocytes and neutrophils in the BM of control mixed WT:WT and BCAP^−/−:WT BM chimeras reconstituted for >8 weeks; plots are representative of 3 independent experiments. Numbers represent frequency of CD45.1⁺ or CD45.2⁺ cells within indicated gated population. (B) Ratio of CD45.2⁺/CD45.1⁺ cells from WT:WT and BCAP^−/−:WT mixed chimeras; data pooled from 2 independent experiments. Inflammatory monocytes and neutrophils were identified as in supplemental Figure 1. (C) Ratio of CD45.2⁺/CD45.1⁺ cells from WT:WT and BCAP^−/−:WT mixed chimeras; data pooled from 2 independent experiments. Follicular mature B cells were identified as CD19⁺B220⁺CD21^hiCD23⁻ live cells and marginal zone B cells as CD19⁺B220⁺CD21^intCD23⁺ live cells. Panels B and C show mean ± standard error of the mean; each symbol represents data from an individual mouse, with n = 11 mice per group, and dotted lines represent a 1:1 reconstitution ratio. *P < .05, **P < .01, ***P < .001, and ****P < .0001. n.s., not significant, as determined by 2-tailed, unpaired Student t test.