Accelerated differentiation of BCAP^−/− CMP cells in vitro. (A) A total of 5000 CMP cells from WT lineage⁻ BM were cultured with SCF, IL-3, and IL-6 for up to 24 hours for maturation into GMP cells. Histogram overlay of CD16/32 expression on WT CMP cells at indicated times; data are representative of 2 independent experiments. (B) Frequency of CD16/32^hi cells at times indicated in panel A from WT and BCAP^−/− CMP cultures; data are representative of 2 independent experiments; data show mean of n = 3 mice per group; for all time points, standard deviation < 3%. *P < .05, **P < .01, and ***P < .001, as determined by 2-tailed, unpaired Student t test.