Increased proportion of IL-6Rα+ cells among BCAP−/− HSPCs. (A) Representative histogram overlay of IL-6Rα expression on LSK, CMP, and GMP cells in lineage− (Lin−) BM of WT mice. Data are representative of 4 independent experiments. (B) (Top) Representative histogram overlays of IL-6Rα expression on LSK (left), CMP (middle), and GMP (right) cells from Lin− BM of WT and BCAP−/− mice; data are representative of 4 independent experiments. (Bottom) Frequency of IL-6Rαhi cells within LSK (left), CMP (middle), and GMP (right) populations from Lin− BM of WT and BCAP−/− mice; data are pooled from 4 independent experiments, and each symbol represents an individual mouse; graphs show mean ± standard error of the mean, with n = 12 mice per group. In panels A and B, isotype control antibody staining is shown as purple shaded histograms. *P < .05, **P < .01, and ***P < .001, as determined by 2-tailed, unpaired Student t test.
