Frequency and phenotypic/morphologic characterization of CD66b⁺LDNs and CD66b⁺NDNs from GDs. (A) Frequency of CD66b⁺ LDNs within CD45⁺ PBMCs from HDs (n = 44) and GDs (n = 53). Graph values indicate medians from independent experiments. Each symbol stands for a single HD or GD. ***P ≤ .001, by the Mann-Whitney U test. (B-D) Representative fluorescence-activated cell sorter (FACS) plots displaying CD16 and CD11b expression in CD66b⁺ NDNs from HDs (B) or CD66b⁺ LDNs (C) and CD66b⁺ NDNs (D) from GDs. (E-G) Representative FACS plots (left column) displaying CD10 and CD16 expression in CD66b⁺ NDNs from HDs (E) or CD66b⁺ LDNs (F) and CD66b⁺ NDNs (G) from GDs. Representative May-Grünwald Giemsa stained cytospins (right column; scale bar = 10 μm) of sorted CD16⁺ (R1) cells from CD66b⁺ NDNs from HDs (E) or CD66b⁺ LDNs (F) and CD66b⁺ NDNs (G) from GDs. Examples of segmented neutrophils (▼), band cells (*), and metamyelocytes (○) are reported.