Frequency and phenotypic/morphologic characterization of mature CD10⁺and immature CD10⁻ neutrophils within CD66b⁺LDNs and CD66b⁺NDNs from GDs. (A-B) Frequency of CD10⁺ and CD10⁻ cells within CD66b⁺ LDNs in PBMCs from GDs (n = 53) (A) or within CD66b⁺ NDNs from HDs (n = 44) and GDs (n = 53) (B). Graph values indicate medians from independent experiments. Each symbol stands for a single HD or GD. ***P ≤ .001, by the Mann-Whitney U test. (C-E) Representative FACS plots of CD11b and CD16 expression and May-Grünwald Giemsa stained cytospins (scale bar = 10 μm) of CD66b⁺CD10⁺ (R1) or CD66b⁺CD10⁻ (R2) cells within CD45⁺ granulocytes from HDs (C) or GDs (E) and CD45⁺ PBMCs (D) from GDs. Examples of segmented neutrophils (▼), band cells (*), metamyelocytes (○), and myelocytes (●) are reported.