CD66b⁺CD10⁻LDNs from GDs increase proliferation and IFNγ production by T cells. (A-B,E-F) CD3/CD28-stimulated T cells were cultured for 96 hours in the absence or the presence of either CD10⁺ NDNs from HDs and CD66b⁺CD10⁻ LDNs from GDs (A,E-F), or CD10⁺ NDNs from HDs and CD66b⁺CD10⁻ NDNs from GDs (B), at a 5:1 neutrophil-to-T-cell ratio and with or without 10 µg mL⁻¹ anti-CD18 or isotype control mAbs (E-F). The percentages of increase/decrease of proliferation, as measured by BrdU incorporation (A-B,E) or IFNγ production (F), by T cells is reported. Graph values indicate medians from independent experiments (n = 4-10). *P ≤ .05; **P ≤ .01; ***P ≤ .001, by the Mann-Whitney U test. (C) CD10⁺ NDNs from HDs and CD66b⁺CD10⁻ LDNs from GDs were cultured for the times indicated either alone (left panel) or in the presence of CD3/CD28-stimulated T cells (right panel) at a 5:1 neutrophil-to-T-cell ratio. The frequency of apoptotic neutrophils is reported. Graph values indicate medians from independent experiments (n = 4-6). *P ≤ .05; **P ≤ .01, by the Mann-Whitney U test. (D) Untreated (negative control) or CD3/CD28-stimulated T cells were cultured for the times indicated either alone (positive control) or in the presence of CD10⁺ NDNs from HDs or CD66b⁺CD10⁻ LDNs from GDs added at a 5:1 neutrophil-to-T-cell ratio. The percentage of apoptotic T cells is reported. Graph values indicate medians from independent experiments (n = 3-7). *P ≤ .05, by the Mann-Whitney U test.