Ampd3^T689A has elevated erythropoiesis. (A) Representative plots showing the flow cytometry gating strategy using CD44 and TER119 staining. Proportion of TER119⁺ erythroblast populations in the (B) bone marrow and (C) spleen, calculated relative to the number of TER119⁻ cells, for WT, Ampd3^T689A/+, and Ampd3^T689A. I, RBCs; II, reticulocytes; III, polychromatic erythroblasts; IV, basophilic erythroblasts; V, proerythroblasts. (D) EPO concentration in the plasma of WT, Ampd3^T689A/+, and Ampd3^T689A. Black bars represent median. (E) Mean ± SEM percentage peripheral reticulocytes in WT (n = 4), Ampd3^T689A/+, (n = 8), and Ampd3^T689A (n = 5) mice over the course of 11 days. Values from Ampd3^T689A have been adjusted so that the peaks of reticulocytosis align. (F) Fold change compared with day 0 in number of CD71⁻ RBCs on day 1-7 after phenylhydrazine (PHZ) injection for WT (n = 5), Ampd3^T689A/+ (n = 4), and Ampd3^T689A (n = 4). Values are average ± SEM. *P < .05; **P < .01; ***P < .001. P values calculated using the Student t test.