Examination of changes in Bcl-2 family proteins and ER stress response proteins after treatment with Ara-C and Ida. Atg7 knockdown cells (ShAtg7) or nonsilencing scrambled control (ShControl) OCI-AML3 AML cell lines were treated with Ara-C (2 μM) or Ida (5 ng/mL) for indicated time intervals, and total lysates were immunoblotted to detect biochemical markers of ER stress (A and D), regulation of apoptosis (B and E), and alteration of Bcl-2 family proteins (C and F). Similarly, immunoblotting detected DNA damage (G) in indicated time intervals in response to Ara-C and Ida. β-Actin was used as a loading control.