T-cell clone 4G11 recognizes endogenously processed and presented BOB1₄₄. (A) Shown are histograms of T-cell clone 4G11 or a control T-cell clone VSU specific for cytomegalovirus-derived peptide TPRVTGGGAM (pp65_TPR). T-cell clones were stained with pMHC tetramer BOB1₄₄:B7 or pp65_TPR:B7. (B-C) T-cell clone 4G11 was cocultured with K562-B7 pulsed with (B) titrated concentration of BOB1₄₄-peptide or (C) 3 BOB1-expressing HLA-B7⁺ B-LCLs. Shown are means with standard deviations of 1 experiment carried out in duplicate. GM-CSF, granulocyte-macrophage colony-stimulating factor.