TCR-transduced CD8⁺ T cells proliferate upon antigen encounter. (A-B) PKH-labeled transduced CD8⁺ T cells were cocultured with irradiated HLA-B7⁺ stimulator cells. Histograms show TCR-transduced (red line) or mock-transduced (blue area) CD8⁺ T cells after 5 days of coculture. (A) Stimulator cells included cell line UM9 and primary ALL, CLL, and MCL. Negative controls included culture in absence of stimulator cells (no stimulators) or coculture with BOB1⁻ K562-B7 cells. Positive control included stimulation in the presence of CD3/CD28 T-cell activator beads (CD3/CD28 beads). (B) Autologous CD40L-stimulated B cells or phytohemagglutinin-activated T cells were used as stimulator cells.