![Figure 2. No early incorporation of EPCs in experimental models of GVHD and IBD. (A) Number of proliferating (Ki67+) ECs in colon of SYN and ALLO mice at day+2 after BMT (n = 3 per group). White arrow marks proliferating EC (4′,6-diamidino-2-phenylindole [DAPI+] [blue], Ki67+ [red-A555] in direct contact with CD31 [green-A488] signal). White asterisks mark proliferating crypt cells. (B) Representative pictures of green-A488 CD31 (vessel-related), DsRed (BM-related), and merged signals of colon and skin sections from SYN and ALLO mice at day+2 after BMT (DsRed+C57BL/6→B6D2F1). (C) Representative pictures of green-A488 CD31, DsRed, and merged signals of colon sections from DsRed+ BM chimeras at day+4 after DSS colitis induction. (D) Representative projections of Z-stacks of colon samples at day+4 after DSS induction from colitis-bearing and control mice, which received DsRed+ EPCs. Green A488-CD31 signal is vessel-related. Scale bar, 30 µm (A-C). Error bar indicates mean ± SEM. *P < .05 by Student t test (2-tailed).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/129/14/10.1182_blood-2016-08-736314/4/blood736314f2.jpeg?Expires=1735587343&Signature=z0vWBX4aeJoXow~ucybVYmd2bUEFW9EAacE-HRtjK9PmHy1T236dEiUbDSFG64iC37x7TdZ0jnvsPhjMyEKrdcvC36yFqRStxq3Rl9LCPEX5uIz2XFKuxFxgown8Pge~TS6Cw-OwCwTQEUG~B2Eom6IxN54TH-979eQx~powVcOcz1msN9c3C2yji91UHAZJjSb5NrWckob9UrGujCwhxy2Ow4dVqYvafa6c-nm-lH1G8XFP-M5PIoJ5t39Nle7r5yZ1bukAT5gOVUFCUTRBFNwAiKMYnvBN3-Riqe9cPfLPW0y~mX6iiZmrV-MTIk4hx-zoW3UjT6axh~Rao5SO7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
No early incorporation of EPCs in experimental models of GVHD and IBD. (A) Number of proliferating (Ki67+) ECs in colon of SYN and ALLO mice at day+2 after BMT (n = 3 per group). White arrow marks proliferating EC (4′,6-diamidino-2-phenylindole [DAPI+] [blue], Ki67+ [red-A555] in direct contact with CD31 [green-A488] signal). White asterisks mark proliferating crypt cells. (B) Representative pictures of green-A488 CD31 (vessel-related), DsRed (BM-related), and merged signals of colon and skin sections from SYN and ALLO mice at day+2 after BMT (DsRed+C57BL/6→B6D2F1). (C) Representative pictures of green-A488 CD31, DsRed, and merged signals of colon sections from DsRed+ BM chimeras at day+4 after DSS colitis induction. (D) Representative projections of Z-stacks of colon samples at day+4 after DSS induction from colitis-bearing and control mice, which received DsRed+ EPCs. Green A488-CD31 signal is vessel-related. Scale bar, 30 µm (A-C). Error bar indicates mean ± SEM. *P < .05 by Student t test (2-tailed).
