The Vegfa/VEGFR2 pathway and adhesion molecules are not upregulated during the initiation of angiogenesis in GVHD target organs. (A) Kaplan-Meier curve and GVHD scoring of B20-4.1.1 (Vegfa inhibitor) and rat IgG2-treated control mice (LP/J→C57BL/6) (n = 8 per group). (B) Kaplan-Meier curve of DC101+MFI (VEGFR1+2 inhibitor) and rat IgG–treated control mice (C57BL/6→BALB/c) (n = 12 per group). Untreated, only BM cell transplanted mice served as internal control (n = 5). Vegfa and VEGFR2 expression in GVHD target organs liver, colon, and skin of ALLO vs SYN mice (n = 5 per group) at day+2 after BMT (C-D) as well as in liver at day+15 after BMT (E). (F) MHC-II and CD31 double-positive area of CD31-positive area in liver of SYN and ALLO mice day+2 and +15 after BMT (n = 7 per group). (G) Expression of adhesion molecules Intercellular Adhesion Molecule 1 (Icam1), vascular cell adhesion molecule 1 (Vcam1), E-selectin, and P-selectin in MACS-sorted liver ECs from SYN vs ALLO mice at day+2 after BMT (n = 5). In panels A-B, P values were calculated by using the log-rank test. In panels C-E,G, gene expression levels were normalized to Gapdh expression and are shown relative to gene levels of a reference untreated sample. Error bars indicate mean ± SEM. *P < .05; **P < .01; ***P < .001; n.s. not significant by Student t test (2-tailed).