Early modifications in ECs have significant functional consequences as indicated by profoundly higher deformation in RT-DC. (A) RT-DC setup and measurement principle (inset shows top view of constriction). (B) Representative images of liver ECs from ALLO (green square) and SYN (blue square) mice at day+2 after BMT (LP/J→C57BL/6) acquired by RT-DC. (C) RT-DC measurements. Scatter plot of deformation vs cell size (cross-sectional area) of 1510 cells (dots). Color indicates a linear density scale. Density contour plots (50%) of ALLO vs SYN mice. (D) Mechanical phenotyping of liver ECs of ALLO and SYN mice at day+2 after BMT (LP/J→C57BL/6). Mean values n = 3. Deformation P = .0002, size P = .285 (***P ≤ .001) by a likelihood ratio test. Images are analyzed for cell size and cell shape (red contours). Scale bar, 5 µm. CMOS, complementary metal-oxide-semiconductor; LED, light-emitting diode.