Platelet removal or inhibition does not affect parasitemia in resolving, non-eCM Pca LV infection and does not affect activation of protective immunity. Parasitemia for each group denoted by filled symbols (left y-axis) with corresponding platelet counts/mL in open symbols (right y-axis) (A-B). (A) Aspirin- (triangle) and saline-injected (circle) groups (n = 5) of Pca-infected mice. (B) Platelet-depleting anti-CD41 mAb (triangle) or isotype control (circle) injected on days –1, 1, 3, and 6PI. (C) Secondary parasitemia after injection of 1 × 10⁷Pca on day 0PI into the anti-CD41 mAb and isotype control groups of mice that had resolved their primary infection; a group of uninfected mice with primary parasitemia is infection control. (D) Primary parasitemia in CD40KO mice that do not resolve Pca infection after IV injection of WT platelets on day −1 and 0PI and in intact mice that resolve their Pca infection. (E) Secondary parasitemia after injection of 1 × 10⁷Pca into 3 groups of mice in panel D; a group of uninfected mice with primary parasitemia is infection control. Average value ± standard error of the mean (SEM) are reported. *P < .05. The experiment in panel A was repeated twice, and in panel B was repeated once.