G-CSF preferentially mobilizes dormant HSCs. (A) Experimental schematic. Mice were placed on dox for 12 weeks and were treated with G-CSF either 4 days or 2 weeks before analysis. BM, spleen, and PB HSPCs were analyzed for GFP label distribution. (B) Histograms of GFP distribution across HSC (BM) and HSPC (spleen and PB) compartments without, 3 hours after, and 2 weeks after G-CSF treatment. (C) Quantification of HSPCs found across GFP gates. Data are represented as mean ± SEM of 6, 7, and 4 mice for control, 3-hour, and 2-week treatments, respectively. Statistical significance was determined by comparing control with 3-hour and 2-week data. *P < .05, **P < .01, ***P < .001 by Welch’s t test.