PPARγ inhibition by GW9662 increases Stat5a and downstream targets. Lin⁻ splenocytes from Se-S BCR-ABL mice treated with or without GW9662 were analyzed for RNA and protein expression of Stat5a and downstream targets. (A) Lin⁻GFP⁺c-Kit⁺Sca-1⁺ FACS-sorted LSCs were treated with or without sodium selenite (500 nM) for 24 hours and the expression of Stat5a and Cited2 was examined by real-time PCR. (B) qPCR expression as fold change compared with Se-S vehicle for each gene and normalized to 18S rRNA expression (n = 4). (C) Western immunoblot analysis showing representative blot and densitometry (normalized to Se-S for each protein and relative to Gapdh) (n = 3-4). Bars represent biological mean ± SEM. All analysis was done in technical triplicate. *P < .05; **P < .01.