Figure 3.
Inhibition of PPARγ represses expression of Ptges-1 and Txas1 in Se-S CML progenitor cells. Lin− splenocytes from Se-S BCR-ABL mice treated with or without GW9662 were analyzed for RNA and protein expression. Se-S mice were given daily i.p. injection of GW9662 (1 mg/kg) starting 1 day prior to BCR-ABL transplant until euthanized (n = 4). (A) qPCR expression as fold change compared with Se-S vehicle for each gene and normalized to 18S rRNA expression. (B) Western blot analysis showing representative blot and densitometry (normalized to Se-S for each protein and relative to GAPDH). Bars represent biological mean ± SEM. All analyses were done in technical triplicate. *P < .05; **P < .01.