Changes in surface receptors of CD8⁺ DCs after TLI conditioning or injection of apoptotic spleen cells. (A) Shows representative FACS patterns and (B) mean percentages of Tim-4⁺, CD1d⁺, CD40⁺, or Rae-1⁺ cells on gated splenic CD8⁺CD11c⁺ cells from untreated wild-type (n = 8) or Jα18^−/− (n = 8) or from TLI conditioned wild-type (n = 8) or Jα18^−/− (n = 8) mice at day 5 after completion of conditioning. Bars show mean percentages. (C) Compares the MFI values ± standard error (SE) for staining of CD8, Treml4, DEC205, SIRPα, and PDL-2 receptors on gated CD8⁺CD11c⁺ spleen cells from either untreated BALB/c mice (noninjected) (n = 5), BALB/c mice given a single IV injection of whole spleen cells from untreated BALB/c donors (Norm cell injection) (n = 5), or BALB/c mice given a single IV injection of whole spleen cells obtained immediately after 10 daily doses of TLI (apoptotic cell injection) (n = 6). Staining of recipient spleen cells was performed 48 hours after the injection of donor syngeneic spleen cells.