Increased NKT-cell expression of PD-1 and NKG2D after TLI is attenuated in CD8⁺ DC–deficient mice. Representative 2-color FACS patterns for CD1dtet (tetramer) vs TCRαβ staining from untreated wild-type (n = 8) or Batf3^−/− (n = 5) or TLI-conditioned wild-type (n = 11-16) or Batf3^−/− (n = 8-9) mice are shown in panel A. Patterns for CD1dtetramer vs PD-1 or NKG2D staining are shown in panels C and E, respectively. The mean percentages of NKT cells among all T cells, mean percentages of PD-1⁺ cells among NKT cells, and mean percentages of NKG2D⁺ cells among NKT cells in the spleen are shown in panels B, D, and F, respectively.