American Society of Hematology

Figure 4.

$Figure 4. Aid loss does not cooperate with Tet2 loss in hematopoietic regulation. (A) The percentage of PB granulocyte/monocyte lineage (Mac1/Gr1), lymphoid lineage (B220/CD3), and hematopoietic progenitor fraction (cKit+) derived from WT, Aid−/−, Tet2del/del, and Aid−/−Tet2del/del adult mice around 10 months old. The data are mean ± SD (n = 3 for each arm). (B) Methylcellulose serial CFU assay using BM cells from WT, Aid−/−, Tet2del/del, and Aid−/−Tet2del/del mice. Absolute number of colonies is shown at different platings. The data are mean ± SD (n = 3 for each arm). (C) The number of CFU-E colony derived from WT or Aid−/− BM cells in first plating. The data are mean ± SD (n = 3 for each arm). (D) Donor BM cells were collected from either WT, Aid−/−, Tet2del/del or Aid−/−Tet2del/del adult mouse (1.0 × 106 lysed BM cells per genotype, CD45.2+, 8 weeks old), mixed with support BM cells (1.0 × 106 lysed BM cells, CD45.1+, 8 weeks old) and injected to lethally irradiated recipient mice (CD45.1, n = 6 for each genotype). Percentage of donor-derived cells (CD45.2+ cells) in PB of primary recipients (left) and secondary recipients (right). The data are mean ± SD. Asterisks indicate the P value: *(P = 7.0736 × 10−9 [WT vs Tet2del/del], P = 8.6984 × 10−8 [Aid−/− vs Tet2del/del], P = 4.0864 × 10−7 [WT vs Aid−/−Tet2del/del], P = 7.4401 × 10−6 [Aid−/− vs Aid−/−Tet2del/del]); **(P = 1.3386 × 10−6 [WT vs Tet2del/del], P = 5.9805 × 10−8 [Aid−/− vs Tet2del/del], P = 4.5634 × 10−6 [WT vs Aid−/−Tet2del/del], P = 3.9577 × 10−7 [Aid−/− vs Aid−/−Tet2del/del]); ***(P = 3.0646 × 10−6 [WT vs Tet2del/del], P = 2.2681 × 10−6 [Aid−/− vs Tet2del/del], P = 1.0519 × 10−5 [WT vs Aid−/−Tet2del/del], P = 1.5423 × 10−5 [Aid−/− vs Aid−/−Tet2del/del]); ****(P = .0023 [WT vs Tet2del/del], P = .0020 [Aid−/− vs Tet2del/del], P = .0030 [WT vs Aid−/−Tet2del/del], P = .0030 [Aid−/− vs Aid−/−Tet2del/del]); *****(P = 2.8775 × 10−7 [WT vs Tet2del/del], P = 1.8491 × 10−6 [Aid−/− vs Tet2del/del], P = 1.4464 × 10−6 [WT vs Aid−/−Tet2del/del], P = 1.0054 × 10−5 [Aid−/− vs Aid−/−Tet2del/del]); ******(P = 8.9093 × 10−7 [WT vs Tet2del/del], P = 8.1382 × 10−7 [Aid−/− vs Tet2del/del], P = 1.6057 × 10−6 [WT vs Aid−/−Tet2del/del], P = 1.4062 × 10−6 [Aid−/− vs Aid−/−Tet2del/del]).$

Aid loss does not cooperate with Tet2 loss in hematopoietic regulation. (A) The percentage of PB granulocyte/monocyte lineage (Mac1/Gr1), lymphoid lineage (B220/CD3), and hematopoietic progenitor fraction (cKit⁺) derived from WT, Aid^−/−, Tet2^del/del, and Aid^−/−Tet2^del/del adult mice around 10 months old. The data are mean ± SD (n = 3 for each arm). (B) Methylcellulose serial CFU assay using BM cells from WT, Aid^−/−, Tet2^del/del, and Aid^−/−Tet2^del/del mice. Absolute number of colonies is shown at different platings. The data are mean ± SD (n = 3 for each arm). (C) The number of CFU-E colony derived from WT or Aid^−/− BM cells in first plating. The data are mean ± SD (n = 3 for each arm). (D) Donor BM cells were collected from either WT, Aid^−/−, Tet2^del/del or Aid^−/−Tet2^del/del adult mouse (1.0 × 10⁶ lysed BM cells per genotype, CD45.2⁺, 8 weeks old), mixed with support BM cells (1.0 × 10⁶ lysed BM cells, CD45.1⁺, 8 weeks old) and injected to lethally irradiated recipient mice (CD45.1, n = 6 for each genotype). Percentage of donor-derived cells (CD45.2⁺ cells) in PB of primary recipients (left) and secondary recipients (right). The data are mean ± SD. Asterisks indicate the P value: *(P = 7.0736 × 10⁻⁹ [WT vs Tet2^del/del], P = 8.6984 × 10⁻⁸ [Aid^−/− vs Tet2^del/del], P = 4.0864 × 10⁻⁷ [WT vs Aid^−/−Tet2^del/del], P = 7.4401 × 10⁻⁶ [Aid^−/− vs Aid^−/−Tet2^del/del]); **(P = 1.3386 × 10⁻⁶ [WT vs Tet2^del/del], P = 5.9805 × 10⁻⁸ [Aid^−/− vs Tet2^del/del], P = 4.5634 × 10⁻⁶ [WT vs Aid^−/−Tet2^del/del], P = 3.9577 × 10⁻⁷ [Aid^−/− vs Aid^−/−Tet2^del/del]); ***(P = 3.0646 × 10⁻⁶ [WT vs Tet2^del/del], P = 2.2681 × 10⁻⁶ [Aid^−/− vs Tet2^del/del], P = 1.0519 × 10⁻⁵ [WT vs Aid^−/−Tet2^del/del], P = 1.5423 × 10⁻⁵ [Aid^−/− vs Aid^−/−Tet2^del/del]); ****(P = .0023 [WT vs Tet2^del/del], P = .0020 [Aid^−/− vs Tet2^del/del], P = .0030 [WT vs Aid^−/−Tet2^del/del], P = .0030 [Aid^−/− vs Aid^−/−Tet2^del/del]); *****(P = 2.8775 × 10⁻⁷ [WT vs Tet2^del/del], P = 1.8491 × 10⁻⁶ [Aid^−/− vs Tet2^del/del], P = 1.4464 × 10⁻⁶ [WT vs Aid^−/−Tet2^del/del], P = 1.0054 × 10⁻⁵ [Aid^−/− vs Aid^−/−Tet2^del/del]); ******(P = 8.9093 × 10⁻⁷ [WT vs Tet2^del/del], P = 8.1382 × 10⁻⁷ [Aid^−/− vs Tet2^del/del], P = 1.6057 × 10⁻⁶ [WT vs Aid^−/−Tet2^del/del], P = 1.4062 × 10⁻⁶ [Aid^−/− vs Aid^−/−Tet2^del/del]).

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