Promyelocytic extracellular chromatin enhances plasmin generation and fibrin structure. (A) Plasma containing plasminogen and isolated concentrated extracellular chromatin were prepared. t-PA and the plasmin substrate Spectrozyme-PL were then added and the absorbance of the liberated p-nitroaniline was continuously measured at 405 nm (OD405). The figure shows mean values of triplicate measurements from 6 independent experiments. Clot lysis assays were performed in normal plasma supplemented with isolated concentrated extracellular chromatin in the presence of 1 nM t-PA. Changes in clot structure were measured by observing clot turbidity at 405 nm (B); lysis time was recorded (C). Each point represents mean ± SD for triplicate samples of independent experiments. *P < .05, **P < .01.
