Fibrin formation is dependent on TF and independent of NET formation. (A) Fibrin staining in TF antibody (21E10) or isotype control (IgG2a)-treated mice following IVC stenosis over 6 hours. (B) Quantification of fibrin deposition (n = 5-6; 3-4 visual fields per mouse; mean ± SD). Scale bar: 100 μm. Consecutive measurements were evaluated by 2-way ANOVA followed by Bonferroni posttests. ***P < .001. (C) Fibrin formation in the stenosed IVC of PAD4^−/− mice and WT mice over 6 hours. (D) Quantification of fibrin deposition. Scale bar: 100 μm (n = 5-6; 3-4 visual fields per mouse; mean ± SD). Consecutive measurements were evaluated by 2-way ANOVA followed by Bonferroni posttests. h, hour; NET, neutrophil extracellular trap; n.s., not significant.