IL-4/CXCL12 loop in a model of inducible ectopic lymphoid organs in mice. (A) RT-qPCR analysis of mRNA transcript for Il4 in wild-type (WT) mice on days 0, 5, 8, 15, and 23 days post cannulation (p.c.), normalized to Pdgfrß. Relative expression was calibrated to 0 day p.c. salivary glands. *P < .05; **P < .01 vs day 0. Results represent 2 experiments with 4 to 6 glands analyzed per group. (B) RT-qPCR analysis of mRNA transcript for Cxcl12 in WT mice, Il4^−/− mice, and Il4r^−/− mice on days 0, 5, 8, 15, and 23 p.c. normalized to Pdgfrß. Relative expression was calibrated to 0 days p.c. salivary glands. *P < .05; **P < .01 vs day 0. Results represent 2 experiments with 4 to 6 glands analyzed per group and are represented as the mean ± SD. Relative expression was calibrated to 0 day p.c. salivary glands. *P < .05; **P < .01.