Stenosis-induced platelet recruitment is reduced in the absence of CLEC-2 or after neutralization of podoplanin. IVC stenosis was applied to Clec1b^fl/fl × Rosa26^+/creERT2 and their Clec1b^fl/fl littermates for 6 hours. Fluorescently labeled syngeneic platelets were infused, and their deposition on the IVC wall was visualized by intravital microscopy. (A) Representative averaged images of adhered platelets (bright white). (B) Percentage of area covered by immobilized platelets, n = 3 to 4. Data are presented as mean ± standard error of the mean. (C) The same experimental design was applied to WT mice injected with anti–podoplanin neutralizing antibody (100 μg per mouse, IV) or IgG control before surgery. (D) Percentage of area covered by recruited platelets; n = 4 for both groups.