Effect of PKal on tPA-mediated intracerebral hemorrhage transformation after ischemic stroke in mice. (A) Representative coronal sections of brain mice at 24 hours after stroke. Red color indicate hemoglobin associated with hemorrhage transformation within the ischemic area in mice treated with tPA and the decreased hemorrhage associated with PKal inhibition on tPA-induced postischemic brain. At 2 hours after stroke onset, mice received tPA (10 mg/kg) either alone of following a 15 minutes pretreatment with BPCCB (1, 3, 10 mg/kg) or saline vehicle. Klkb1^−/− mice were treated intravenously with saline or tPA (10 mg/kg). (B) Volumes of intracerebral hemorrhage were quantified with hemoglobin assay at 24 hours after stroke. Data are presented as mean ± SEM, n = 6-8. **P < .01; ***P < .001. (C) Quantitative analysis of brain edema at 24 hours after ischemic stroke. Data are presented as mean ± SEM, n = 7-8. *P < .05; **P < .01; ***P < .001. Numbers in x-axis labels for panels B and C indicate concentrations of BPCCB in milligrams per kilogram.