Tregs inhibit TRALI-associated physiological responses. (A) Rectal temperatures of naive BALB/c mice or mice first treated (+) or not (−) with the indicated antibodies for Treg depletion and then injected with the TRALI-inducing antibody 34-1-2s or indicated control antibodies. (B) Pulmonary PMN percentages in the BALB/c mice treated in panel A. (C) MPO activity in the lung tissue of Treg-depleted BALB/c mice treated in panel A. (D) Plasma MIP-2 levels of BALB/c mice treated in panel A. All mice were analyzed 90 minutes after the second injections. For the statistical analyses, only significant comparisons of interest are shown. Panels A-D were analyzed with a 1-way ANOVA with Tukey’s post hoc test. Each dot represents 1 mouse, and error bars represent SD. *P < .05, **P < .01, ****P < .0001.