PMNs and ROS are required for antibody-mediated TRALI induction. (A) Lung W/D weight ratios of WT C57BL/6 mice or gp91phox KO mice which first underwent in vivo depletions of CD4⁺ T cells and/or PMN by the indicated antibody injections (+) or not (−) and were then injected with the TRALI-inducing antibody cocktail 34-1-2s + AF6-88.5.5.3 or indicated control antibodies. (B-E) Rectal temperatures (B), plasma MIP-2 levels (C), pulmonary PMN percentages (D), and lung MPO enzyme activity (E) in CD4⁺ T cell-depleted WT C57BL/6 mice with or without PMN depletion or gp91phox KO mice injected (+) or not (−) with the indicated antibodies. All mice were analyzed 90 minutes after the second injections. For the statistical analyses, only significant comparisons of interest are shown. All panels were analyzed by a 1-way ANOVA with Tukey’s post hoc test. Each dot represents 1 mouse, and error bars represent SD. *P < .05, **P < .01, ****P < .0001.