cDKO mediates p53 pathway and IFN system activation in HSPCs. (A) Heat map of commonly up- and downregulated transcripts in cDKO MEP and GMP from microarray analysis. (B) Networks enriched in cDKO MEP and GMP by ingenuity pathway analysis of protein–protein interaction databases only; network name: Cell Cycle, Antimicrobial Response, and Inflammatory Response. (C) Top 4 overlapping canonical signaling pathways and P values for enrichment in cDKO MEP and GMP. (D-G) GSEA of RNA-seq analysis of LKE⁺CD48⁻ cells showing loss of stemness signature (D), p53 pathway activation (E), and interferon activation (F-G) in cDKO HSCs. See also supplemental Figure 4. FDR-qval, false discovery rate q value; NES, normalized enrichment score.