High frequency and dominance of clone with integration on chr21 in patient 301. (A) IS-specific ddPCR clonal tracking of dominant clone in patient 301. (B) Total and IS-specific ddPCR VCN measurement in sorted lineages. (C) NKG2C⁺ population expansion in patient 301 compared with another ADA trial patient and 2 healthy donors. Whole blood was stained with CD3, CD19, CD11b, CD56, and NKG2C antibodies and analyzed via flow cytometry. A CD56-FITC vs NKG2C-PE plot is shown for CD3⁻CD19⁻CD11b⁻ cells gated for characteristic lymphoid forward and side scatter. (D) EBV copy number correlates with NK cell count in patient 301. EBV copy number was determined in PBMC samples via ddPCR for the BALF5 gene.